Vitamin D-dependent Calcium-binding Protein
نویسندگان
چکیده
منابع مشابه
Vitamin D-dependent Calcium-binding Protein
A method of purification of a vitamin D-dependent calciumbinding protein from chick intestinal mucosa is described. The purification procedure involved the use of ammonium sulfate precipitation, gel filtration on Sephadex G-100, and preparative disc electrophoresis. The assay system for calcium-binding protein is based upon the competition between calcium-binding protein and an ion exchange res...
متن کاملIntestinal membrane calcium-binding protein. Vitamin D-dependent membrane component of the intestinal calcium transport mechanism.
A particulate fraction of rat intestinal mucosal homogenates, termed the "calcium-binding complex," contains three vitamin D-dependent activities: calcium binding of high affinity, calcium-dependent adenosine triphosphatase, and p-nitrophenylphosphatase. These particulate activities vary concordantly with intestinal calcium transport, suggesting that they represent membrane components of the tr...
متن کاملPlacental calcium binding protein in rats. Apparent identity with vitamin D-dependent calcium binding protein from rat intestine.
Rat placenta contains a calcium binding protein (CaBP) which is closely related or identical with the vitamin Ddependent CaBP in rat intestine. Placental CaBP was detected using antiserum prepared in rabbits against the intestinal form of the protein purified as previously described (Bruns, M. E. H., Fliesher, E. B., and Avioli, L. V. (1977) J. Biol. Chem. 252, 4145-4150). The antiserum was mon...
متن کاملAnalysis of vitamin D-dependent calcium-binding protein messenger ribonucleic acid expression in mice lacking the vitamin D receptor.
To investigate the roles of the receptor-dependent actions of 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3] in the regulation of vitamin D-dependent calcium-binding proteins (calbindin-D), the messenger RNA (mRNA) levels of calbindin-D9k and -28k were examined in vitamin D receptor (VDR)-ablated mice and control littermates. In VDR-ablated mice, calbindin-D9k mRNA was dramatically reduced in the inte...
متن کاملStructure and nucleotide sequence of the rat intestinal vitamin D-dependent calcium binding protein gene.
The vitamin D-dependent intestinal calcium binding protein (ICaBP, 9 kDa) is under transcriptional regulation by 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3], the hormonal active form of the vitamin. To study the mechanism of gene regulation by 1,25-(OH)2D3, we isolated the rat ICaBP gene by using a cDNA probe. Its nucleotide sequence revealed 3 exons separated by 2 introns within approximately 3 ki...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1968
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)92040-5